Figure 5. Correlative image analysis and the mining of KEGG database identified the biology signaling pathways in the morphogenetic domains (central body position). (A) We identified 20 distinct gene expression patterns by computer image clustering analysis in the projected feature space of Principal Component Analysis. (B) We performed GO term analysis and identified 14 highly expressed biology signaling pathways from the gene expression image clusters. We generated 14-bit binary barcode that indicates the “presence” – “absence” state of the pathways in detection (white color: “presence;” black color: “absence”). We then performed hierarchical clustering and grouped the gene expression patterns according to pathway barcode similarity. By correlative analysis of the gene expression and the anatomy patterns, we identified the cell populations that highly express the biology signaling pathways. We performed pairwise comparison with the barcodes and constructed the map of the morphogenetic domain signaling network. For instance, along the A-P axis, we detected the mTOR signaling pathway in cluster 12 (neuron) but not cluster 17 (muscle). The mTOR signaling pathway regulates a wide variety of cell Biology Processes, such as lipid metabolism, autophagy, protein synthesis, ribosome biogenesis, cytoskeletal organization and cell survival [26]. The results of analysis along the other 2 body axes of V-D and L-R were presented in the supplementary Figure 7.