Supplementary Figure 10: The morphogenetic domain signaling network model suggested that the Notch and the Ephrin signaling pathways cross-talk and play important roles in regulating tissue morphogenesis. (A-C) The Notch and the Ephrin signaling pathways were highly active during embryo early organogenesis. The components of the pathways showed regional specificity of gene expression in multiple morphogenetic domains. (N and E stand for Notch and Ephrin signaling pathway components, respectively in the circle shape markers. The white color indicates “detected” and black color indicates “not detected.”) (D) We identified the differentially expressed genesets in morphogenetic domains by clustering and correlative image analysis. Results of GO term analysis indicate that the Wnt, Notch and Ephrin pathway components were enriched for the genesets. The three pathways, Wnt (blue), Notch (red) and Ephrin (dark blue) crosstalk with each other through intermediate hub genes (orange circle). To the center of the network, the genes, plx-2, sma-6, mes-1 and unc-129, in the orange circle, have the interactions with all the three pathways of Wnt, Notch, Ephrin pathways while the three genes, nhr-66, sup-17 and ego-1, in the cyan circle have interactions with Notch and Ephrin signaling pathway components only. The crosstalk through the intermediate hub genes among signaling pathways is essential for coordinate tissue morphogenesis. The mutation of the gene sup-17, regulating Ephrin and Notch signaling pathway crosstalk, cause embryonic lethality. While the mutation of mes-1, the intermediate hub gene of all the three pathways, gives rise to phenotypes of elongation defect, symmetric division of germline cells P2 and P3, no Intestine.