Supplementary Figure 7: Correlative image analysis and the mining of KEGG database identified the molecular signaling pathways in the morphogenetic domains (central position of sectioning) We identified 20 distinct gene expression patterns by graphics image clustering analysis (clusters shown in the order of left-to-right, top-to-bottom. (A) Along the A-P axis, we detected the mTOR signaling pathway in cluster 12 (neuron) but not cluster 17 (muscle). The mTOR signaling pathway regulates a wide variety of cell Biology Processes, such as lipid metabolism, autophagy, protein synthesis, ribosome biogenesis, cytoskeletal organization and cell survival. (B) Along the V-D axis, the ECM-receptor signaling pathway was detected in Cluster 2 and 18 (muscle) but not cluster 17 (pharynx). Cell adhesion plays the important roles in regulating the collective cellular behaviors in proliferation, differentiation, migration, apoptosis and the assembly of polarized tissues. The mutation of the extracellular matrix (ECM) receptor encoding gene, PAT-2/PAT-3 integrin [28], leads to failed epithelial morphogenesis and defects in embryo body elongation. (C) Along the L-R axis, we identified TGF-β pathway in cluster 5, 12 and 16 (hypodermis, intestine and pharynx). The TGF-β signaling pathway plays the critical role in body size regulation.